Diabetic erectile dysfunction (ED) is a common complication of type 2 diabetes mellitus. The pathophysiology of ED is closely associated with the toxic effects of hyperglycemia, which leads to an elevation of reactive oxygen species (ROS) and promotes the secretion of pro-inflammatory cytokines. Prolonged exposure to high glucose levels causes monocytes to become proinflammatory, resulting in the expression and secretion of interleukin-10 (IL-10), which in turn reduces glucose uptake in an autocrine and paracrine manner, transforming monocytes into an anti-inflammatory phenotype. Umbilical cord mesenchymal stem cells (MSCs), known for their immunoregulatory properties, have gained significant interest as a potential strategy for regulating functional cells and tissues, including oxidative stress in ED. This study aims to demonstrate the ability of MSCs to regulate oxidative stress through IL-10 in ED. A total of 20 male Sprague-Dawley rats (6 to 8 weeks old) were randomly divided into four groups: healthy group, negative control group, MSCs 1x106 (T1 group), and MSCs 3x106 (T2 group). After a 16-hour fast, 20 rats were randomly selected and intraperitoneally injected with streptozotocin to induce diabetes mellitus (DM). At 8 weeks after STZ injection, rats with ED were identified based on their unresponsiveness to erectile stimulation within 30 minutes. After MSCs treatment, the rats were sacrificed, and blood samples were prepared for examination. The level of IL-10 in ED showed a significant difference compared to the healthy group. MSCs treatment increased the IL-10 levels in the T1 and T2 groups up to 727.93± 19.09 and 675.54±9.27, respectively. The increase in IL-10 levels was supported by a dose-dependent decrease in blood glucose levels. MSCs have the potential to regulate ED by increasing the levels of the anti-inflammatory cytokine IL-10.