Effective infection control practices and collaboration between healthcare professionals are essential for ensuring patient safety. This study aimed to explore the current infection control practices and collaboration between nurses and sterilization technicians in healthcare settings. A total of 150 participants, including 100 nurses and 50 sterilization technicians, completed a questionnaire assessing their adherence to infection control guidelines and the level of collaboration. The results indicated a high level of knowledge regarding infection control guidelines among both groups. Nurses demonstrated higher compliance rates with hand hygiene and personal protective equipment guidelines compared to sterilization technicians. Sterilization technicians reported a higher level of collaboration with nurses in infection control efforts. However, nurses identified barriers to collaboration, such as a lack of effective communication and limited time for collaboration. These findings underscore the need for ongoing education and training to ensure consistent adherence to infection control guidelines. Interventions should focus on enhancing collaboration, improving communication, and fostering a culture of teamwork between nurses and sterilization technicians. Addressing these barriers can contribute to improved infection control practices and patient safety outcomes in healthcare settings.