The objective of this study is to predict and understand the interactions of phosphorylated flavin compounds with RfBP and their potential to enhance RfBP function, with the aim of designing novel and more effective ligands for RfBP with a focus on therapeutic applications. In the absence of Crane RfBP's 3D structure, its protein sequence was retrieved from the NCBI database in FASTA format. SWISS-MODEL employed this sequence as the query for comparative modeling, facilitating 3D structure prediction. The Ramachandran plot gauges protein or experimental structure quality, categorizing amino acids into favorable, allowed, or disallowed regions