Asparagus racemosus is one of the most extensively used traditional medicinal plant in India. The therapeutic properties of A. racemosus are due to the presence of secondary metabolites like quercetins. This study aims to measure the levels of quercetins in several Asparagus racemosus genotypes’ tissues, including the roots, shoots, and leaves. HPLC-DAD method was optimized for the quantification of quercetins. In roots, shoots, and leaves, quercetin levels ranged from 0.043 mg/ml to 0.276 mg/ml, 0.033 mg/ml to 0.257 mg/ml, and 0.046 mg/ml to 0.293 mg/ml, respectively. For quercetin, the LOD and LOQ values were 0.048 and 0.146, respectively. Insights on the variety of quercetin content across distinct genotypes and plant parts are provided by the current study. Additionally, it was shown that leaves contain more quercetins than roots and shoots