Background: Spinal stenosis may result in compression of the spinal cord and cause symptoms associated with cervical myelopathy symptoms. Space available for the cord (SAC) can be used as an indicator of spinal stenosis. This study evaluates the reference value of SAC by MRI from C3 to C7 vertebrae. Materials and methods: A cross-sectional study was conducted in the Department of Radiology, Santosh Medical College, Ghaziabad, Uttar Pradesh, in collaboration with the Department of Radiology, Hind Institute of Medical Sciences, Barabanki, Uttar Pradesh. MRI-based study on the midsagittal diameter of the spinal canal, spinal cord and its SAC value of the lower cervical spine (C3-C7) were done from 320 subjects (160 males and 160 females) of 18 to 59 years of age. Results: The mean value of the sagittal diameter of the spinal canal from C3 to C7 was 14.50 mm ± 1.24 in males and 14.18 mm ± 1.12 in females. The mean value of sagittal diameter of the spinal cord from C3 to C7 was 6.01 mm ± 0.69 in males and 5.85 mm ± 0.63 in females. The SAC value was 8.48 mm ± 1.22 in males and 8.33 mm ± 1.12 in females.