Introduction: The most common intensive care unit (ICU) acquired infection among patients receiving mechanical ventilation is ventilator-associated pneumonia (VAP). Accurate clinical and microbiological identification of VAP is crucial for both choosing the right antibiotics and avoiding antibiotic overuse. VAP is associated with prolonged duration of mechanical ventilation and ICU stay. The estimated attributable mortality of VAP is around 10%, with higher mortality rates in surgical ICU patients and in patients with mid-range severity scores at admission. Methods: The present study was a prospective and descriptive study carried out in the Department of Microbiology and the ICU at a Tertiary care centre for a period of 2 years i.e, June 2021 to June 2023 . A total of 200 consecutive patients who were hospitalised in the ICU were included in the study. The patient’s consent was duly taken and epidemiological clinical study was carried out. The OpenVAP was assessed in 200 patients who were mechanically ventilated and admitted to the ICU. To identify VAP, the Clinical Pulmonary Infection Score (CPIS) was employed and the microbiological profile was assessed. The organism was isolated and identified by the use of different biochemicals test followed by the Antimicrobial susceptibility testing by Kirby Bauer disc diffusion method following the CLSI guidelines 2021.