Although Indian health care system is currently operating within an environment of rapid social, economic and technical changes and hospitals is an integral part of health care system. Accreditation would be the single most important approach for improving the quality of hospitals. As a result staff working in emergency department needs to focus on NABH guidelines and protocol. This study identifies the knowledge & practice regarding NABH protocol followed in emergency department. This study was cross - sectional. The study includes Staff Nurses, Physician, and Paramedical Staff & Supportive Staff. The total sample size is 450. A self-administered and self-structured questionnaire was created specifically for the purpose of the study to collect data. Using Microsoft excel 2010, a descriptive analysis was performed to determine the knowledge & practice regarding NABH protocol followed in emergency department. All participants had some knowledge regarding NABH protocol. Although there was individual variation practice and knowledge regarding NABH guidelines and protocols. It is evidence that the knowledge towards NABH delegation among the staff nurses, physicians, paramedical staff and supportive staff to gain further information and guidelines for further developments of knowledge and chops in practice.