Background: It is well recognized that dietary acidic load directly or indirectly affects overall health in many aspects. One's food habit is responsible for the dietary acidic load by producing acid or alkaline precursors. Naturopathy is an alternative system of medicine which highly emphasizes lifestyle changes for disease prevention and management. One of its basic principles is to consume more naturally available food like fruits and vegetables and avoid food from animal sources and processed food helping the body to maintain an appropriate acid-base balance. This study aims to evaluate the urine pH of patients admitted to a residential naturopathic hospital before and after the naturopathic diet intervention of 1 week. Methods: Every patient [n=35] who got admitted from February 2022 to April 2022 was recruited for this prospective singlecentre single-arm pre-post study to evaluate the effect of a naturopathic diet on urine pH, considering the inclusion and exclusion criteria. A one-week naturopathic diet intervention was given to the patients. The urine pH was measured before and after the naturopathic diet intervention, as urine pH reflects our body's net endogenous acid production [NEAP]. Many clinical trials and reviews describe the importance of dietary acidic load in disease prevention and management. However, no study portrays the importance of the naturopathic diet principle in disease prevention and management relating to dietary acidic load. Results: There was a significant difference in the mean outcomes of the group [p=0.007]. The intervention increased the urinary pH after seven days. Conclusion: A naturopathy diet can positively affect health by maintaining the urine pH at appropriate levels and preventing our body from many metabolic conditions.