Objectives: This study evaluated the effectiveness of vitamin E (vit E) supplementation in diabetic rabbits in decreasing their hyperglycemic condition and oxidative stress (O.S.) status. Materials and Methods: 10 albino rabbits were randomly selected and made diabetic by administrating Alloxan. Out of 10 diabetic rabbits, five were treated with insulin. Another ten healthy rabbits were selected and grouped into 2, each having five rabbits. Out of four groups (5 rabbits in each group), three groups were treated with vit E (80 mg/kg/day) for six months. The rest of one group of rabbits was kept as healthy control. Their weight, glycaemic parameters like fasting blood sugar (FBS), postprandial blood sugar (PPBS), glycosylated haemoglobin (HbA1c) and oxidative stress parameters like serum vitamin C and malonaldehyde (MDA) levels were estimated. All parameters were tested at the beginning of the study and re-evaluated after six months of treatment. Result: There was a significant decrease in FBS and PPBS in all the vit E treated groups, but an insignificant decrease in HbA1c level was found. In all vit E treated groups significant increase in serum vitamin C and a decrease in serum MDA was found. Conclusion: So vit E protects against oxidative injury associated with diabetic conditions.