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ISSN 2063-5346
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EVALUATION AND QUANTIFICATION OF PIPERINE IN SHRINGYADI CHURNA -AN AYURVEDIC FORMULATION USING RP-HPLC

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C. Sumithra1, S.V. Suresh Kumar2*, L. Siva Sanker Reddy3, R. Nageswara Rao4, S. Muneer5, K. Maheshwari6, C. Madhsudhana Chetty7
» doi: 10.48047/ecb/2023.12.si10.00208

Abstract

Objective: The project was aimed at developing an analytical method to quantify piperine in shringyadi churna- an Ayurvedic formulation by RP-HPLC technique. Methods: The RP-HPLC technique was carried to work out Various parameters like, loss on drying, extractive values, powder properties like angle of repose, bulk density, Hausner’s ratio, compressibility index etc. and analytical method and inspecting consistency of the method by performing the different validation parameters like system suitability, specificity, linearity, accuracy, precision, LOD, LOQ, Robustness and assay. The column used was Inertsil ODS Column C18 (4.6×250mm) 5μm of Shimadzu. The HPLC was Shimadzu make with PDA detector and model 20AD. Results: In the course of stream lining the analytical method, we have used the mobile phase Methanol and 0.1% formic acid (0.1ml of formic acid dissolve in 100ml 0f water) in the ratio of 80:20. The drug was detected at 344 nm on UV-Visible spectrophotometer. The retention time was at 5.69 min with the run time of 8 min. The linearity range of piperine was from 0.5 μg/ml to 10μg/ml and the Regression coefficient calculated to be (R2) 0.997. The corresponding recognition limits (LOD and LOQ) of the Avanafil was 2μg/ml and 10μg/ml respectively. Precision studies were carried out and the RSD values were found to be less than two. The degradation studies were successfully conducted. Conclusion: The significant advantages were reduction of retention time, the lower limit in linearity being at least 10 times less and the mobile phase used was quite cheaper than the reported methods. The method is also sensitive, reproducible, quick and economical.

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