The present study was designed to evaluate the anti-inflammatory, analgesic and antipyretic activities of the aqueous and ethanolic extracts of roots of Mitragyna parvifolia. Albino mice were used as experimental animals to evaluate these activities. The study was performed in three phases; Phase-I for evaluation of anti-inflammatory activity, Phase-II for antipyretic and Phase-III for analgesic activities were evaluated. Carrageenan induced paw edema, brewer yeast induced pyrexia and acetic acid induced writhing methods were used to evaluate antiinflammatory, antipyretic and analgesic activities, respectively. Tests were performed by dividing the animals in five groups. First group was negative control, second group was positive control, third, and fourth and fifth groups were treated with 125, 250 and 500 mg/kg of extracts respectively. The data were statistically analyzed using ANOVA where p < 0.05 were considered significant. The results suggested that roots of Mitragyna parvifolia possess anti-inflammatory, analgesic and antipyretic activity