The aim of the present study is to evaluate the anti-urolithiatic activity of ethonolic extract of Amaranthus blitum in ethylene glycol induced urolithiasis in rats. Urolithiasis is a condition that occurs when these stones exit the renal pelvis and move into the remainder of the urinary collecting system, which includes the ureters, bladder, and urethra.Amaranthus blitum linn belongs to the family Amaranthaceae is selected for the study due to its phytochemicals like flavonoids, carotenoids, β-carotene, vitamin C, phenolic compounds, and natural antioxidant . Healthy Male Wistar rats (150-200 gms) were selected and divided into five groups having six animals in each. Group-I served as normal and received regular rat food and drinking water adlibitum. Ethylene glycol (0.75%) & Ammonium chloride (1%) in drinking water was fed in Group-II to Group-V for induction of renal calculi for 28 days. Group-III received standard antiurolithiatic drug Cystone (750 mg/kg b. wt.). Group-VI and Group- V received ethanolic extract of Amaranthus blitum 200 mg/kg b. wt. and 400 mg/kg b. wt. for 28 days. Extracts were given once daily by oral route. Finally based on improvement in urine and serum parameters, antioxidant parameters, it is concluded that the ethanolic extract of leaves of Amaranthus blitum possess antiurolithiatic activity