Candida albicans, Trichophyton rubrum, Microsporum canis, Epidermophyton floccosum are the most important species responsible for infections with invasive fungal disease. Resistance of these fungi species to antifungal drugs has led scientists to pay more attention to traditional medicine herbs. Due to the limitations in the treatment of fungal diseases such as shortages, high prices and drug resistance or reduced susceptibility to fungal drugs we decided to study the antifungal effect of novel herbal emulsion. Aim: The purpose of this study was to evaluate the antifungal activity of novel herbal drug emulsion against most common fungal strains by implementing modern diagnostic technique. Methodology: Novel herbal drug emulsion made by two different methods named as sample A and sample B (decoction method) using different concentrations (50% 75% and 100 %) The antifungal activity was determined by using agar well diffusion method. Zone of inhibition of samples were compared with that of standard drug Ketoconazole 5 % w/v (Positive control) Result: Drug showed significant biological activity against all fungal strains. Conclusion: In vitro findings of this study confirmed antifungal activity of Novel herbal drug emulsion on common fungal pathogens.