Antimicrobial activity is the most important to inhibit the growth of bacteria and fungi infections, prevent the creation of microbial colonies, and destroy microorganisms. These have been widely used and greatly benefited the health-related quality of human life. However, the rapid emergence of antibiotic-resistant bacteria has created various problems with serious trouble for the medical community in measuring ionizing radiation exposure in dosimeter applications. Therefore, the use of doped nanoparticles as an alternative for measuring antimicrobial activity has been investigated. In this paper, the Agar disc-diffusion antibiotic susceptibility method is developed for antimicrobial activities analysis. The proposed technique uses the input of the samarium-doped, silver-doped, and Europium-doped CeO2 nanoparticles. Prepared samples are tested for measuring the antimicrobial activities against the ciprofloxacin bacteria and Itraconazole yeast or fungus by applying an Agar disc-diffusion antibiotic susceptibility method. In order to find the antibacterial and antifungal effects of the doped CeO2 nanoparticles, methanol is used as a control in the disc-diffusion susceptibility test. During the disc-diffusion process, the zone of inhibition is measured based on the compound on Clostridiumper fringens, Neisseria gonorrhoeae,