Cnidoscolus aconitifolius is commonly known as Tree Spinach and it belongs to the family Euphorbiaceae. Chaya possesses excellent medicinal properties for the treatment of different ailments. The different parts of the Cnidoscolus aconitifolius plant including leaves, seeds, latex, and fruit exhibited to have medicinal value. This research focuses on the antioxidant and anti-bacterial activity of leaves of Cnidoscolus aconitifolius. Here, we examine the study that has been conducted on this remarkable plant. We suggest chaya as a promising crop due to its simplicity of growing, potential yield, and most importantly its significant nutritional value. The leaves of the Chaya plant were collected from nearby regions dried, powdered, and extracted with ethanol. We studied the identification of antioxidants using (DPPH) 2, 2-Diphenyl-1-picrylhydrazylradical scavenging activity in Cnidoscolus aconitifolius, as Cnidoscolus aconitifolius is an important herbal plant. The 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) method is a popular, fast, simple, and inexpensive method for measuring the antioxidant activity of compounds. It uses free radicals to evaluate whether substances have the capacity to act as hydrogen suppliers or freeradical scavengers (FRS).