BACKGROUND: Diabetes mellitus is a chronic disorder that is associated with cardiovascular complications, renal dysfunction and various types of diseases including metabolic syndrome. The present study aimed to investigate the key biochemical parameters such as serum uric acid, vitamin D, vitamin C, magnesium and calcium along with lipid profile that are associated with insulin resistance in type-II diabetes mellitus (DM) patients in Kadapa area. MATERIAL AND METHODS: The present case-control study was conducted at Fathima Medical College, Kadapa. We recruited 100 type-II diabetic patients and 100 healthy volunteers in the present study. The age groups of the study subjects were between 40 and 70 years in Kadapa urban area. Five ml of fasting blood samples and 3 ml of whole blood samples were collected in the clinical biochemistry laboratory for the estimation of fasting blood sugar (FBS), 2-hour postprandial blood sugar (PPBS) by GOD-POD method along with serum uric acid, magnesium and calcium by IFCC approved fully automated clinical chemistry analyzer. Lipid profile namely total cholesterol, triglycerides, HDL, LDL and VLDL were estimated by the CHOD-PAP and GPO methods. Serum levels of vitamin C vitamin D were estimated by 2.4 dinitrophenyl hydrazine method and CMIA method, respectively.