Objectives: The following study comparatively evaluates changes in mechanical properties of Gutta Percha cones in herbal solutions using aloe vera gel, lemon grass oil and pancha tulsi, keeping sodium hypochlorite as the control group and antimicrobial efficacy of these solutions against the most predominant microorganism of root canal, E.Faecalis. Materials and Methods: 200 F3 Gutta Percha cones were used for the study. GP cones were treated with the herbal solutions and NaOCl, group 1- lemon grass oil, group 2- pancha tulsi, group 3- aloe vera gel and group 4- sodium hypochlorite, which was then subjected to test for tensile strength and percentage elongation under Universal Testing Machine. Antimicrobial efficacy was checked with the help of formation of zones of inhibition on the agar culture plates incubated with E.faecalis by dispensing herbal solutions and NaOCl. Decontamination of GP was done by noting the absence or presence of turbidity. Results: The Mechanical strength of GP did not alter much in group 1 as compared to that of NaOCl. Antimicrobial efficacy was seen in lemon grass oil with highest zones of inhibition. Conclusion: All extracts proved to be potential rapid chairside disinfectants of GP cones and can be used as root canal irrigants, with LG showing the highest antimicrobial activity and good in tensile strength and percentage elongation.