Background: Diabetes mellitus (DM) is a cluster of metabolic disorder with various complications that significantly impact patient’s health outcomes. Hyperglycemia associated with DM can increase oxidative stress which is hypothesized to play a significant role in the development of DM and its complications. When the oxidative stress increases in diabetes, methylglyoxal (MG), a strong and hazardous reactive metabolite that is derived from glucose and fructose metabolism is accumulated in blood, further increases oxidative stress and also affects insulin secretion from pancreatic β-cells. This study aimed to evaluate the relationship between MGO and oxidative stress with DM. Method: Total 130 participants were included in the study and divided into two groups as healthy control group (65) and diabetic group (65) irrespective of gender. Diabetic patients with complications were excluded from the study.