OBJECTIVE: The present study is to evaluate hepatoprotective activity of ethanol extract of Dioscorea bulbifera L. on CCl4 Induced liver damage on experimental rats. METHODS: The ethanol extract of Dioscorea bulbifera L. (Dioscoreaceae) was investigated for CCl4 Induced hepatotoxicity. Silymarin was used as a reference drug at a dose of 50 mg/kg b.w. All the experimental animals were divided into 5 groups of 6 each. In this study, hepatoprotective effect of the two different doses (200 and 400 mg/kg b.w. p.o) of EEDB will be investigated for 28 days to evaluate dose dependent activity. Effect of EEDB on biochemical parameters, liver homogenate enzymes and also for histopathological studies. RESULTS: All the values show significant activity over hepatoprotective activity of EEDB on consumption of the extracts, with greater effect exhibited by the 400 mg/kg extract. At the end of the experiment the results of biochemical analysis and histopathological studies show significant changes on compared with disease control group. CONCLUSION: From the results, it was concluded that EEDB have hepatoprotective activity. Further studies will be needed in future in order to determine which one or more of its active constituents have the main effects.