“Osteoarthritis (OA)” affects millions of individuals globally and is a common and crippling ailment. One of the most prevalent types of OA is knee OA, which is frequently accompanied with pain and disability. A non-invasive therapeutic method for OA has been presented, called “low level laser therapy (LLLT)”. Methods: The purpose of this study was to assess the impact of LLLT on knee OA pain and disability scores. For this trial, 50 people with knee OA in total were enrolled. For four weeks, the participants received LLLT three times per week. The “Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC)” and the “Knee Injury and Osteoarthritis Outcome Score (KOOS)” were used to calculate the pain and disability scores both before and after the treatment. Results: Following LLLT therapy, the scores for pain and disability significantly improved. The KOOS score increased from 41.2± 10.1 to 71.3± 8.3 (p< 0.001), whereas the WOMAC score increased from 54.4± 8.9 to 29.1± 7.3 (p< 0.001). These findings show a statistically significant reduction in knee OA symptoms following LLLT therapy. Conclusion: For knee OA, LLLT is a successful non-invasive therapeutic alternative. In patients with knee OA, it can considerably lower pain and disability scores. The effects of LLLT over the long term on knee OA require further research.