Introduction: The study was performed in beta thalassemia major patients to evaluate liver parameters. Beta-thalassemia is most common hereditary hematologic disorder characterized by severely impaired beta-globulin synthesis. Repeated blood transfusion in beta thalassemia major patient is highly challenging for many developing countries where the disease is prevalent, representing a major and unsustainable health burden. Aim: This study aims to investigate liver parameters in patients diagnosed with beta-thalassemia major. Materials and Methods: The study comprises a total of 80 subjects between age group of 4 to 14 years including patients with beta-thalassemia major (n=40) and healthy volunteer (n=40). Blood samples were collected and serum was separated to be tested for aspartate aminotransferase, alanine aminotransferase and total bilirubin were done by Erba XL-640 fully automated analyser. Results: The levels of aspartate aminotransferase, alanine aminotransferase and total bilirubin in serum were found to be extremely statistically significant (p<0.05) in beta-thalassemia major patients than in healthy volunteer. Conclusion: Repeated blood transfusions in beta thalassemia major patients causing significant liver damage that results in elevated levels of serum aspartate aminotransferase, alanine aminotransferase. Serum total bilirubin can be a good marker for monitoring bile duct obstruction resulted from hemolysis and blood transfusion.