The aim of this study was to compare the marginal accuracy and internal fit of the provisional crowns made with manually fabricated technique, CAD/CAM milling technology, and 3D printing technology. Materials and Methods: one mandibular left first premolar (#35) resin tooth was prepared and duplicated using 3D printing technique to produce twenty-one resin dies. Accordingly, a total of (N=21) provisional crowns were constructed. The Constructed provisional res¬torations were divided into 3 groups according to the method of construction (n=7 for each group), Group 1: Provisional crowns were constructed using conventional manual technique, Group2: Provisional crowns were constructed using CAD/CAM milling technique, Group3: Provisional crowns were constructed using 3D Printing technique. All Provisional crowns were bonded to their corresponding resin dies by us¬ing CharmTemp ZONE temporary cement and subjected to thermocycling procedure simulating approximately one month of clinical situations Results: Statistical analysis showed that, for marginal gap distance, the highest value was found in conventional group (67.30±11.78), followed by 3D Printing (43.63±5.85), while the lowest value was found in CAD/CAM (27.21±2.80).103.23). ANOVA test showed that there was a significant difference between different groups (p<0.001). on the other hand, for total internal gap distance results, the highest mean value was recorded in CAD/CAM group (52.72±3.25), followed by 3D Printing group (52.06±2.65), while the lowest value was recorded in conventional group (39.09±3.41). ANOVA test showed that there was a significant difference between different groups (p<0.001). Conclusion: Within the limitations of the current study, CAD/CAM constructed provisional crowns have a superior marginal accuracy values compared to 3D Printed and conventional crowns. While regarding to the internal fit, conventional provisional crowns recorded the highest internal adaptation values (occlusal and axial fit) compared to CAD/CAM and 3D Printed crowns.