The phytochemical investigation's capacity to separate and submit the active ingredients in Vigna mungo (L.) Hepper seeds to further pharmacological testing can be of considerable use to researchers and the field of traditional medicine. The purpose of this study was to investigate the potential of an aqueous extract of Vigna mungo (AEVM) seeds (fabaceae) to shield rats' liver and kidneys from rifampicin-induced damage. Following selection, eight groups of six albino rats, weighing 200–250 grammes apiece, were created. Four groups were assigned hepatoprotective activity and four groups were assigned nephroprotective activity. Normal control was given to Group 2 and AEVM treatment was given to Group 3. Standard medication treatment was given to Group 2. Group 4 was given normal medication therapy. Additionally, the nephroprotective impact was investigated. The results are corroborated by function, histology, biochemistry, and physical features. The medications used for hepatoprotective action include rifampicin, silymarin, and SGPT, SGOT, ALP, and BIT diagnostic kits. Serum BUN, serum creatinine, and serum uric acid for nephroprotective effect. To extract the powdered Vigna mungo seed, water was utilised. Some preliminary phytochemical research was done to identify the phytoconstituents. The hepatoprotective and nephroprotective effects of AEVM were assessed in rats administered with rifampicin to cause hepatotoxicity and nephrotoxicity. After one-way analysis of variance (ANOVA), multiple comparison tests utilising the "Tukey-Kramer" method are conducted. The AEVM identified proteins, phytic acid, total phenolic compounds, alkaloids, carbohydrates, flavonoids, glycosides, and tannins. Rifampicin caused significant alterations in the following areas: biochemical (increase in serum glutathione pyruvate transaminase (SGPT), oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP) and total bilirubin (BIT) level, increase in blood urea nitrogen (BUN), serum creatinine, and serum uric acid level), functional (barbiturates-induce sleeping time), physical (increased liver weight, decreased body weight), and histological (damage to hepatocytes, nephrons). Pretreatment with AEVM significantly reduced the physical, biochemical, and histological changes that rifampicin caused in the liver and kidney, respectively.