Hyperglycemia is a chronic medical condition that occurs when the body is unable to properly regulate blood sugar levels. This condition is commonly associated with diabetes, both type 1 and type 2, when the body either does not produce enough insulin or is unable to use insulin effectively. Insulin is a hormone that helps regulate blood sugar by allowing cells to absorb glucose for energy refers to high levels of glucose in the blood. Untreated or poorly managed diabetes can lead to serious complications, including heart disease, stroke, kidney disease, nerve damage, and eye problems.. Type 2 diabetes mellitus (T2DM) is commonly regarded as a severe public health problem due to its potentially lethal implications and increased mortality risk. Reactive oxygen species (ROS) generation and antioxidant defence are in balance in Oxidative stress, which allows the body to detoxify its negative effects and prevent cell damage. Hyperglycemia generates reactive oxygen species (ROS), which in turn cause many types of cell damage. Secondary problems in diabetes mellitus ultimately occur from cell damage. The evaluation of oxidative stress markers in type 2 diabetic patients was the primary goal of the current investigation, 150 diagnosed Type 2 DM patients with no systemic complication and co-morbidities were chosen as the cases, while 150 healthy volunteers with similar age and sex were chosen as the controls. Catalase (CAT), Superoxide dismutase (SOD), Glutathione Peroxidase (GPx), Reduce Glutathione reductase (GSH), Glutathione reductase (GR) and Protein carbonyl was estimated by colorimetric methods while spectrophotometric analysis was used to determine lipid peroxidation. Significant increase (P < 0.05) were seen as Catalase (CAT), Superoxide dismutase (SOD), Glutathione Peroxidase (GPx), Reduce Glutathione reductase (GSH) ,Glutathione reductase (GR) Lipid peroxidation(LPO) and Protein carbonyl contrasted with the control group in DM patients. The findings of the present investigation revealed that diabetic patients experience higher levels of oxidative stress. It is possible to employ oxidative stress markers as early indicators of diabetic problems. Patients with DM may benefit from new therapeutic approaches that attempt to reduce oxidative stress.