Objective: To study physicochemical, qualitative and quantitative estimation of phytochemicals, determination of total phenol and flavonoid content, antioxidant activity and FT-IR spectral analysis in floral parts of Senna surattensis. Methods: The solvents ethanol, and aqueous are used for extraction of Senna surattensis plant parts. Phytochemical screening of plant parts was carried out in both the solvents. Determination of total phenol content was carried out using Folin - Ciocalteau method and total flavonoid content using Aluminium chloride spectrophotometric method. Antioxidant activity of methanolic extract of plant samples were evaluated with DPPH standard method. The FT-IR is a very useful technique for identifying the functional groups present in the mixture.