Punicagranatum and Carica papaya are two regularly consumed fruits whose photochemical makeup and anti-diabetic properties will be assessed in this study. In vitro experiments were used to measure the antidiabetic activity while several spectroscopic techniques were employed for the photochemical investigation. According to the study's findings, both fruits had a sizable anti-diabetic effect, albeit Punicagranatum had a more potent impact. Several bioactive substances, including flavonoids, phenols, and tannins, were detected by photochemical analysis, which may be the cause of the antidiabetic action that was reported. According to the research, Punicagranatum and Carica papaya ingestion may offer diabetics possible therapeutic advantages. The research study showed that Punicagranatum and Carica papayaexhibited various biological activities. We found that extract showed various primary metabolites presence and showed major biological activity like anti-diabetic activity, Based on the primary metabolites these extracts have anti-diabetic activity. Based on our in-vivo research studies, these extracts play a vital role in pharma to control the diabetics and act as herbal formulations in alternative medical fields.