Background-aim of this study is to evaluate and compare the tensile bond strength with two different adhesive systems on sound and demineralized dentin after using a Papain-based gel (Carie-care) for chemo-mechanical caries removal. Materials And Methods: Forty recently extracted human molars were collected. Teeth were mounted longitudinally up to CEJ in self-cure acrylic and divided into four groups Group 1: Mechanical removal of caries with fifth-generation total-etch adhesive (Adper Single Bond 2, 3M ESPE) Group 2: Mechanical removal of caries with seventh-generation universal adhesive (One Coat 7 universal, Coltene) Group 3: chemo-mechanical excavation of carious dentin was conducted using the papain-based gel with fifth-generation total-etch adhesive (Adper Single Bond 2, 3M ESPE). Group – 4: chemo-mechanical excavation of carious dentin was conducted using the papain-based gel with seventh-generation universal adhesive (One Coat 7 universal, Coltene). Nanohybrid Composite (Filtek™ Z350 XT Universal Restorative) was used to build composite cylinders. A tensile bond strength test was performed on Universal Testing Machine and the modes of failure were determined after sectioning with hard tissue microtome under Scanning Electron Microscope. Results: The data were submitted to two-way ANOVA and Tukeys multiple post hoc tests test (p ≤ 0.05). Irrespective of caries removal method, Adper Single Bond 2 presented significantly higher bond strength than One Coat 7 Universal. Adhesive failures were observed more under scanning electron microscope. Conclusion: The mechanical method of caries excavation resulted in better values of tensile bond strength than the chemo mechanical method with Carie care gel. Total etch bonding agent system proved to be better than all-in-one system.