Root canal management is a necessary procedure because caries spread quickly in deciduous teeth, causing pulpal damage by contamination of the pulpal tissue by bacteria and their toxins. The search for a superior root canal irrigant is ongoing due to the drawbacks of conventional root canal irrigants. Chewing sticks made from Salvadora persica (Miswak), a popular oral hygiene product, have a variety of biological qualities. Aim: to evaluate the antibacterial effect of 12.5% miswak, and 7.5% Neem extracts as novel irrigant solutions in comparison with 0.2% chlorhexidine irrigant solution against Enterococcus Faecalis microorganisms in primary anterior single-rooted teeth. Methods: A total of forty-five extracted single-rooted primary anterior teeth were used in this study, the teeth were taken from children patients (between 2 to 8 years) who lost those teeth due to trauma that resulted in avulsion or intrusion or extraction of primary anterior teeth or due to delayed exfoliation of primary anterior teeth. This study was categorized into three groups; the first group; 12.5% miswak extract, the second group; 7.5% Neem extract as a novel irrigant solution as the “study group”, while, the third group; 0.2% chlorhexidine irrigant solution as a “control group”. Results: all tested irrigant solutions (Miswak, Neem, chlorhexidine) showed a statistically significant antibacterial effect against Enterococcus Faecalis microorganisms. Also, the results of this study exposed a statistically significant difference against Enterococcus Faecalis microorganisms among all tested irrigant solutions. The results showed that the use of 0.2% chlorhexidine irrigant solution showed a statistically significant effect in reducing the Enterococcus Faecalis microorganisms count when compared to 12.5% miswak and 7.5% Neem extracts. Moreover, the results of this study exposed that 7.5% Neem extract had a statistically higher significant antibacterial effect against Enterococcus Faecalis microorganisms when compared to 12.5% Miswak Conclusion: 12.5% Miswak and 7.5% Neem extracts presented significant antibacterial effect against Enterococcus Faecalis microorganisms and can be used as effective alternative irrigant solutions in primary teeth. Chlorhexidine irrigant solution is more effective against Enterococcus Faecalis microorganisms when compared to 12.5% Miswak and 7.5% Neem extracts as irrigant solutions in primary anterior teeth. Chlorhexidine irrigant solution is still the gold standard irrigant solution in primary anterior teeth.