Context- The desire for whiter, younger-looking, healthier teeth has raised the market for dental whitening. Carbamide peroxide is commonly used in home bleaching procedures as it is safer and causes fewer side effects. Strawberries are one of the natural components that are now utilised to whiten discoloured teeth as it contains both ellagic and malic acids. However bleaching agents results in mineral loss resulting in decreased enamel microhardness. After bleaching, remineralizing therapy using a remineralizing chemical like anticay can restore the enamel's lost surface hardness. Aim- The aim of this invitro study was to evaluate the effect of anticay on the microhardness of enamel after bleaching with carbamide peroxide and strawberry extract. Materials and methods- Twenty-five freshly extracted teeth decoronated and divided mesiodistally into two halves to obtain a total of fifty samples. Samples were randomly divided into three groups as follows. Group 1: Control group(n=10) Group 2: (n=20) Subgroup 2A (n=10): Bleaching with 10% carbamide peroxide Subgroup 2B (n=10): Bleaching with 10% Carbamide peroxide and an additional Anticay application for 1 minute after treatment. Group 3: (n=20) Subgroup 3A (n=10): Bleaching with 2% strawberry gel Subgroup 3B (n=10): Bleaching with 2% strawberry gel and an additional Anticay application for 1 minute after treatment The samples were subjected to indentation to test the microhardness using Knoops hardness analyser. Statistical Analysis- Data was analysed using Student t test and ANOVA. p value <0.05. Results- It was observed that Group 1 has maximum microhardness value (369.80) whereas Subgroup 2A shows lowest microhardness value (285.20). A highly significant difference in the values of microhardness was seen between Subgroups treated with and without anticay application. Subgroups subjected to anticay application post bleaching showed higher microhardness value than Subgroups without anticay application. [p - value < 0.001]. Thus, it indicates that application of anticay post bleaching with carbamide peroxide and strawberry gel significantly increased the microhardness when compared to carbamide peroxide and strawberry gel used alone. Conclusion- The application of remineralizing agent containing calcium sucrose phosphate/anticay after bleaching increased the microhardness of enamel.