Medicinal plants constitute an important source of potential therapeutic agents for diabetes and hyperlipidemia. One of the most important strategies for prevention of postprandial hyperglycemia and hyperlipidemia is inhibition of carbohydrate digestive enzymes (α-glucosidase and α-amylase), inhibition of fat digestion and absorption. Aim: The purpose of present study was to investigate the effect of Hydro alcoholic extract (HEMO) of Moringa oleifera on inhibition of alpha-glucosidase and pancreatic alpha-amylase related to diabetes mellitus. Method: In-vitro antioxidant activity and anti- diabetic studies. Results: The IC50 values of HEMO for sucrase inhibitory activity and α-amylase inhibitory activity had been 248.26 ± 0.31µg/ml and 71.35 ± 0.52µg/ml respectively. The study's findings indicated that HEMO shown robust activity almost dose-dependently, indicating that it is a potent -glucosidase inhibitor that is comparable to the gold standard, acarbose. But compared to nojirimycin, it has also been found to exhibit -amylase inhibitory action. Conclusion: These outcomes verify that HEMO exhibits α-glucosidase and α-amylase inhibitory properties. The results provide evidence that Moringa oleifera leaf extract may have the potential to treat diabetes mellitus.