Introduction: Angiogenesis is a key event in cancer pathology. Current treatment targeting angiogenesis in cases of solid tumours is expensive and inaccessible and newer molecules will require a long time to be available in the market. Melatonin has shown potential in inhibiting angiogenesis.Aim:The study aimed to evaluate melatonin's inhibitory effects on angiogenesis in the chick embryo Chorioallantoic Membrane (CAM) model. Methods: This was an in-ovo study on the chick embryo. Melatonin 1mM was compared with a positive control of Timolol 0.01% and a blank control of Dimethyl Sulfoxide (DMSO 99.99%). Results: Melatonin 1mM (0.232 mg/ml) showed a 61% reduction in vessel density and a 20% reduction in vessel length which was significantly greater than DMSO