Approximately a little less than 20 percent of people worldwide are affected with chronic kidney disease (CKD). The body of research over the last ten years has shown that vitamin D is involved in a wide range of actions involving several regulatory mechanisms. The present investigation was therefore carried out to ascertain and evaluate the Vitamin D status in individuals with chronic renal disease in light of the aforementioned facts. Materials & Methods: 120 patients with chronic kidney disease were enrolled. Venous blood drawn from the subjects following 12-14 hours fasting. Serum 25- OH vitamin D estimated by ELISA method. Serum urea, creatinine, calcium, phosphorus, and eGFR calculated by using Cockcroft – Gault formula. The data was entered into the Microsoft excel and the statistical analysis was performed by statistical software SPSS version 25.0. Results: Mean age of the patients was 54.7 years. Mean serum vitamin D levels were found to be 22.58 ng/ml. Out of 120 patients, serum vitamin D levels were sufficient in 25.83 percent of the patients while they were insufficient in 35.83 percent of the patients. Mean serum vitamin D levels were deficient in 38.33 percent of the patients. According to CKD grading, 8.33 percent, 14.17 percent, 29.17 percent and 48.33 percent of the patients were of Grade 3a, Grade 3b, Grade 4 and Grade 5 respectively. While correlation of Vitamin D status and serum phosphate levels with CKD grading analyzing statistically, significant results were obtained. Conclusion: It is still unknown whether vitamin D deficiency is the cause or only the consequence of various chronic diseases. Since these chronic diseases lack specific treatment or the treatment effects are not curative, strategies for the control of chronic diseases should focus on the prevention