Polycystic Ovarian Syndrome (PCOS) is one of the most significant endocrinological disorder among adolescent women. Several biochemical characteristics tend to vary in such patients. It gives a great need in identification and early diagnosis and also benefits in therapeutic outcomes of the patients. Aim and Objectives: The aim of this study is to evaluate various biochemical parameters in PCOS. The objective of this study is to understand the association between bio chemical parameters such as Fasting blood sugar, glycated heamoglobin and lipid profile. Methodology: A cross sectional study was carried out in tertiary care hospital and analysed using case control model. Women above 18 years of age are diagnosed with PCOS using Rotterdam criteria were included in the study. Patients with severe comorbidities were excluded from the study. Results: After initial screening 80 patients were included in control group while 80 patients were cases (PCOS). The mean age was around 21.23±3.14 and average BMI was 27.32±2.16. PCOS patients have an elevated glycemic profile(P<0.0001*), increased LDL (P<0.0001) and triglyceride levels (P<0.0001*) indicating PCOS impact on entire human system. Conclusion: Early detection, monitoring and management of all the biochemical parameters can help in restricting the complications of this condition