The information technology (IT) sector contributes significantly to India's economic development and employment of a huge number of Indians. Employee well-being (EWB) has constantly been discovered to be objectively relevant to all different sorts of industries and employees, and it has developed into a wide area of research. Companies constantly seek profitable expansion through new product development, efficiency, hiring and training good salespeople, and mergers and acquisitions (M&A). In this paper, for the first time, this paper provides a comprehensive study for examining the influence of M & A on Well-being of Employees in Indian IT Industries. For this purpose, based on literature and discussions with experts, a questionnaire containing 33 EWB scale items was developed and the level of agreement of each EWB scale item after M&A was asked to 384 respondents. Afterward, relative agreement index (RAI) is used to analyze the level of agreement of each of 33 EWB scale items after M & A. In addition, principal component analysis is used to shortlist the factors on which the M & A has significant level of agreement. The practical contributions and applications of the study are then discussed in view of EWB of Indian IT employees after M & A.