Ethanobotany has great importance due to its speculative role to cognize plant-human interaction as well as for highlighting the practical applications and contextualized uses of plants. Ziziphus mauritiana is a common fruit tree species in area. In present study leaves and bark of medicinally important plant Ziziphus mauritiana which belongs to family Moraceae, were collected from three different sites for in vitro antioxidant and antimicrobial activity screening. Ethanopharmacological survey was carried out in many areas of District Sargodha, Punjab, Pakistan. According to the survey Ziziphus mauritiana can be effective in curing number of diseases. The ethanol, methanol, aqueous and choloroform solvent extract were used to screen the antimicrobial activity of plant extract against selected pathogens (E.coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pyogenes, candida albicans) at 200mg/ml concentration by disc diffusion method. The results of antibacterial activity were revealed that ethanolic and choloroform extracts remained more effective and exhibits good activity as compared to methanol and aqueous mediums. Canidada albicans did not show any activity. The antimicrobial inhibition of plant extracts were compared with standard antibiotics. Phytochemical analysis were confirmed the presence of antioxidants (Tannins, Sponins, Flavoniods, Terpeniods and Caroteniods) in bark and leaves of Ziziphus mauritiana.