Background: Serum Vitamin D deficiency has emerged as very significant global health concern, affecting diverse populations across various demographic and geographic landscapes. This research aims to comprehensively explore the prevalence, impact, and interventions related to serum Vitamin D deficiency, shedding light on the multifaceted dimensions of this health issue. Aim: The primary objective of our current research is to investigate prevalence of serum Vitamin D deficiency in diverse populations, identify its impact on health outcomes, and assess the effectiveness of existing interventions. By understanding the intricate factors contributing to deficiency, we aim to formulate targeted strategies for prevention and management. Methods: A systematic and comprehensive research analysis will be conducted, incorporating data from diverse populations worldwide. Epidemiological studies, clinical trials, and meta-analyses will be reviewed to evaluate occurrence and impact of serum Vitamin D deficiency. Interventions, including dietary approaches, supplements, and lifestyle modifications, will be evaluated for their efficacy in addressing deficiency. Results: The findings will provide a detailed overview of occurrence rates of serum Vitamin D deficiency in diverse populations, seeing aspects like age, gender, geographical location, and socio-economic status. The impact of deficiency on health outcomes, including musculoskeletal disorders, immune function, and overall well-being, will be elucidated. Additionally, the analysis will present a critical evaluation of various interventions, highlighting their effectiveness in mitigating deficiency and associated health risks. Conclusion: This research contributes to a deeper understanding of the prevalence, impact, and interventions for serum Vitamin D deficiency in diverse populations. The insights gained will inform public health strategies, clinical guidelines, and future research initiatives aimed at preventing and managing Vitamin D deficiency across different demographic groups.