The Caridina pseudogracilirostris shrimp, known for its herbivorous nature and adaptability to both wild and laboratory conditions, has gained popularity as an ornamental fish in aquariums due to its unique features such as transparency, color, and elongated rostrum. Understanding the reproductive biology and embryonic development of this species is crucial for its conservation and aquaculture management. In this study, the researchers focused on the expression pattern of the pax6 protein, which plays a critical role in tissue and organ formation during embryonic development. The embryos at various developmental stages were quantified in wet weights. The results indicated a gradual decrease in protein concentration as the embryos progressed from the cleavage stage to the pre-hatching stage, suggesting that the proteins were being utilized for organogenesis. The researchers then investigated the expression of pax6, a gene known for its involvement in eye development and central nervous system regulation in both vertebrates and invertebrates. Immunohistochemistry using a pax6 antibody revealed the localization and expression pattern of the protein during different embryonic stages. The highest expression of pax6 was observed in the first post-nauplius stage, coinciding with the development of the eyes and nerve ganglia. This finding supports the crucial role of pax6 in organ maturation. The expression of pax6 decreased in the pre-hatching embryo stage, indicating its maintenance function in developed organs. To validate the protein expression levels observed through immunohistochemistry, an enzyme-linked immunosorbent assay (ELISA) was conducted. This quantitative analysis confirmed the protein concentration changes observed during embryonic development. The findings contribute to our understanding of the role of pax6 in invertebrate embryonic development and highlight the need for further investigation into the presence and function of Pax6 in larval and adult stages of this species.