The dominance of MRSA has been linked to infectious diseases that has increased morbidities and mortality in patients. The goal of the present study was assessing the isolation and identification of marine actinomycetes were screened clinical bacterial pathogens.In this study the potential strain KA8 were isolate from Keelkatalai soil sample. The isolated marine actinomycetes were screened for their antibacterial activity by using the Cross-streak method against MRSA, Escherichia coli, Streptococcus pyogenes and Streptococcus pneumoniae.In light microscopy, KA8 strain results showed gram-positive, filamentous rod shape and short chains of spores. The KA8 strain identified as Streptomyces spby genus level identification. Finally the ethyl acetate extract showed the maximum zone of inhibition observed in MRSA (28±1.00) at 100μl concentration.The scavenging activity shows the 25.92μg/g at 100 (μg/ml) concentration.The major compounds are Monoethylmalonate monoamide,Propanal, 2,3-dihydroxy ether,dl-Aspartic acid,N-Hydroxymethylacetamide and N,1-Dimethylhexylamine were found in the ethyl acetate extract of KA8 strain detected by GC-MS analysis. Hence this identified compound will be screened for drug synthesis after purified compound.