In this research paper we are trying to study these elements can effectively tackle the drawbacks of conventional electrolytes used in magnesium-ion batteries. The electrolyte's overall environmental friendliness is enhanced by the biocompatible and sustainable foundation that the cellulose acetate matrix offers. Magnesium perchlorate, as the conductive salt, increases the passage of magnesium ions inside the electrolyte, improving ion conductivity. The rationale behind using DMFO as the solvent is its capacity to efficiently dissolve magnesium perchlorate and cellulose acetate, guaranteeing a uniform electrolyte composition. The magnesium ion conductivity, reduced internal resistance, and enhanced electrochemical stability shown in the data are encouraging and suggest that the electrolyte may be suitable for use in advanced battery applications. Keywords: Magnesium ion conducting biopolymer electrolyte , cellulose acetate , magnesium per chlorate, biopolymer electrolytes, ionic conductivity, highest conducting sample, FTIR, XRD.