Background: Patient safety is a major healthcare challenge. Due to a lack of safety culture knowledge among dental professionals. The aim of this study: To measure the level of patient safety culture using the Safety Attitude Questionnaire (SAQ-C) and identify factors associated with positive attitudes toward patient safety. Materials and methods: A cross-sectional survey was conducted on 272 dental healthcare workers, Includes dentists and dental assistants within the dental departments of hospitals in Makkah, Kingdom of Saudi Arabia. The data for the study were collected between January 2022 and March 2020. The survey (SAQ-C) comprised 32 items and reflects five dimensions of patient safety culture. The second section collects demographic information that supposedly affects attitudes toward patient safety. Logistic regression analyses were used to identify factors that supposedly influenced positive attitudes toward patient safety. Results: Mean SAQ-C scores were significantly higher in respondents who were male, older, dentists, supervisors, and working in clinics. Positive attitudes toward patient safety were found in (55.7%) participants. Multivariate analyses revealed age and place of work are significantly associated with positive safety attitudes. Conclusion: the study provides important information on patient safety attitudes for dental healthcare workers. Also, provides the current status of patient safety culture and helps raise awareness of it. Most notably, the study identified several factors associated with positive attitudes toward patient safety. The information can be used to improve patient safety in the future.