Background: Laboratory services are an essential component of high-quality healthcare systems and are used to detect diseases of public health importance. Patient satisfaction with laboratory services is critical to proper treatment. Patient satisfaction is an important and commonly used indicator to measure and determine laboratory service levels. Numerous studies conducted in hospitals in different countries have shown low patient satisfaction with laboratory services. This study aimed to assess patient satisfaction with laboratory services and related factors at Mecca Medical Center, Saudi Arabia. Methods: A descriptive cross-sectional study was conducted from January to March 2022. Patient satisfaction with laboratory services was assessed using an interviewer-administered questionnaire from a previous similar study. Statistical analysis was performed using SPSS Version Soft 28 software. Bivariate and multivariable logistic regression were used to assess the relationship between the dependent and independent variables. A P value less than 0.05 was considered a statistically significant association. Results: A total of 414 study participants participated. Systematic random sampling was used to select study participants; 221 (53.4%) were female. About 37.4% of the respondents are between the ages of 29 and 38. The majority of study participants, 245 (58.9%), were satisfied with the clinical laboratory services provided, 135 (32.6%) were dissatisfied, and 35 (8.5%) were neutral. Conclusion: Patient satisfaction was good: nearly six out of 10 patients were satisfied with clinical laboratory services. The availability of all prescribed services and the ability to use services without direct payment increase patient satisfaction, but long wait times for services decrease patient satisfaction.