Background: Pregnancy rate is increased with growing endometrial thickness. Intrauterine infusion of plateletrich plasma is a new approach that has been suggested for the treatment of thin endometrium. Objective: The aim of the study was to evaluate the effect of FERTISERATM, a super activated autologous platelet-rich plasma therapy in the endometrial thickness and pregnancy outcomes in south Indian women. Methods: A prospective interventional trial was conducted in 698 patients were enrolled in this study based on the predetermined inclusion criteria from the year 2017 to 2020. FERTISERATM, super activated autologous platelet rich plasma (PRP) was prepared by centrifugation of the patient’s peripheral blood that has been enriched with platelets. A total of 4 mL of FERTISERATM, solution was divided into two equal portions to inject into each ovary. Results: The positive pregnancy rate was 77.65% after FERTISERATM PRP procedure. 8.6% of patients were cancelled cycle and remaining were negative pregnancy. There was a significant increase in the endometrium line thickness after the 2nd dose follow up when compared with baseline (p<0.001). Conclusion: The present study concluded that FERTISERATM PRP procedure can increase the endometrial thickening and receptivity and thusimprove chances of conception.