Since ancient times, acacia catechu has been used in the traditional "Ayurvedic" medicine of India. But no attempt has been made to standardise.In terms of active ingredient or marker, the herbal extract serves as the major component. A chromatographic fingerprint is used to depict the therapeutically desirable chemical components of herbal medicines. This study suggests an exact HPTLC approach for the quantitative assessment of flavonoids like catechin, kaempferol, quercetin, and taxifolin from water, ethyl acetate, and butanol fractions of Acacia catechu extract. According to the research, catechin and taxifolin, two flavanoids, are present in the water fraction of the extract in amounts of 17.80% and 3.48%, respectively. Catechin, kaempferol, and quercetin have respective concentrations in the butanol fraction of the Acacia catechu extract of 15.48%, 3.04%, and 11.79%. Catechin, taxifolin, kaempferol, and quercetin were all present in the ethyl acetate fraction of the Acacia catechu extract at amounts that were, respectively, 28.61%, 1.51%, 2.62%, and 12.32%.