Identification and analysis of toxins present in biological samples has become essential to investigate any case including homicide, suicide, sexual assault cases, bio-crime, etc. Microbial growth phases, mechanism of synthesis, action of toxins can be studied to solve cases. Alongside, typical colonization of microbes in humans varies based on geographical location which helps to determine the actual location of crime and disposal of cadaver if transported. Several techniques have been used to detect toxins/ pathogens at lowest possible concentrations/numbers. The most common methods include Gas Chromatography-Mass Spectroscopy (GC-MS), Fourier Transform- InfraRed Spectroscopy (FTIR), Sequence targeting of biomolecules, etc.