The study aimed to develop microspheres containing Nateglinide, which could be used in the gastrointestinal to enhance the drug's retention and bioavailability. Various polymers, such as carbopol 934, methylcellulose, and eudragit, were used in the solvent diffusion emulsion process to create the microspheres. The micrometric properties, in vitro lightness, percent yield, capturing efficiency, and in vitro tests were evaluated, along with FT-IR and SEM