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ISSN 2063-5346
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FORMULATION AND DEVELOPMENT OF MEDICINAL PLANT BASED DOSAGE FOR TREATMENT AGAINST INFLAMMATORY PROBLEMS

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Prashant Kumar Verma, Mr Kuldeep Singh, Mr. Vikram Singh
» doi: 10.48047/ecb/2023.12.si4.1773

Abstract

The objective of current research work was formulation and development of medicinal plant based dosage for treatment against inflammatory problems. The physical tests conducted on the plant extract of Arnica Montana evaluated its nature, color, odor, and taste. The extract was in the form of coarse powder and had a yellow to orange color. It had a distinct aromatic odor, which is often described as slightly floral and herbal. In terms of taste, the extract was found to be bitter. It is important to note that these organoleptic properties were specific to the arnica extract being evaluated. The extractive values of the plant extract of Arnica Montana were determined for both alcoholic and aqueous solutions. The extractive value in alcohol was found to be 14.75% w/w, indicating the amount of extractable constituents present in the extract when dissolved in alcohol. On the other hand, the extractive value in aqueous solution was determined to be 21% w/w, representing the quantity of extractable constituents in the extract when dissolved in water. These values provide information about the solubility and extractability of the active constituents of Arnica Montana in different solvents. The total ash value of the extract was found to be 12.5% w/w, indicating the percentage of residue left after complete incineration of the extract. The water-soluble ash value, which represents the portion of ash that is soluble in water, was determined to be 8.5% w/w. Additionally, the acid-insoluble ash value, which represents the portion of ash that remains insoluble even after treatment with acid, was found to be 7.2% w/w. The phytochemical screening revealed the presence of saponins and alkaloids in the Arnica Montana extract, while other tested compounds such as steroids, triterpenoids, glycosides, tannins, phenolic compounds, flavonoids, proteins, and carbohydrates were not detected. The anti-inflammatory action of extract showed potential effects in reducing edema in the carrageenan-induced rat model. The standard drug and both treated groups exhibited varying degrees of reduction in edema compared to the control group, indicating the potential anti-inflammatory activity of the formulation. In acute toxicity study no clinical signs, no deaths, remarkable body weight changes or gross necropsy was found

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