The aim of the present work was to prepare an alcohol based herbal hand sanitizer. This sanitizer was prepared by using herbal oils such as eucalyptus oil, lemongrass oil, clove oil and aloe vera gel including other excipients. The sanitizer was formulated by the dispersion method. The excipients which were used included denatured alcohol, Carbopol 940, polysorbate 20, glycerine, triethanolamine and preservatives. This study was aimed to prepare an alcohol based herbal hand sanitizer for antimicrobial purpose. The pH of sanitizer was found to be between 6.56 to 6.67. The viscosity of sanitizer was found to be between 376 to 838. The spread ability was good and found in range between 6.33 to 7.16. The drug content of Eucalyptus Oil in formulations was found to be 89.8% in F1, 93% in F2 and 97.4% in F3 and the drug content of Lemongrass Oil in formulations was found to be 87% in F1, 88% in F2 and 90% in F3. In-vitro drug release study was found to be 52.6% in F1, 35.2% in F2 and 62.65 in F3. The Anti-microbial study of the formulation was done against the standard drug (Streptomycin) and found to be effect and the Stability of the formulations was found to be stable upon storage for 3 months. All these studies have revealed that the formulation F3 is best because it has better pH, Viscosity, spread ability, Drug content, In-vitro drug release study, Anti-microbial study and Stability.