Medicinal plants, plays a predominant role in curing and preventing diseases due to their antiviral and antimicrobial activity against human microorganism. Herbal Mouthwash are in high demand as compare to chemical mouthwash, because they act on mouth pathogen and microbes and reduce the pain instantly and are also has a no more side effects. The most commonly infectious diseases cause by a many pathogens and microbes are Dental carries and periodontal diseases at different stages of their life time. The aim of present work is to formulate and to evaluate itseffectiveness against microbes present in oral cavity. The four herb Azadirecta indica (neem), Mentha longifolia (mint), Syzgium aromaticum (clove) and Ocimum sanctum (tulsi) were selected for mouth wash and Prepared formulation was further evaluated for physical properties like pH, color and stability. The present mouthwash possesses a good antibacterial property. This preparation is stable in different temperature condition Present mouthwash is a liquid preparation which normally contains antibacterial and antiseptic agents. These solutions can be used to reduce the microbial growth and it reduces infection in the oral cavity