The aim of the present investigation is to evaluate the Antiarthritic potential of A. sativum and Z. officinalis extracts mediated glycosomes formulation by a thin film of lipid hydration method. The in vitro anti-arthritic activity of A. sativum and Z. officinale ethanol extract glycerosome was determined by Protein denaturation inhibition and egg albumin denaturation inhibition methods. The protein denaturation inhibition activity of ASEE glycosome formulations F1 and F2 are 60.2±1.02 and 64.8±0.72 %, which are well comparable with the protein denaturation inhibition activity shown by the standard drugs.