This study aimed to formulate and evaluate antifungal microspheres using extracts from Aegle marmelos leaves. Three different types of polymers, namely HPMC, Carbopol 934, and Carbopol 940, were used to develop the microspheres. The microspheres were characterized for various physicochemical parameters such as angle of repose, bulk density, tapped density, Carr’s Index, and Hausner's ratio, which revealed satisfactory flow properties. The phytochemical analysis of Aegle marmelos leaves confirmed the presence of antifungal compounds such as flavonoids, alkaloids, saponins, steroids, and tannins. The in-vitro drug release profile demonstrated sustained release characteristics, with the microspheres formulated using Carbopol 940 showing the most promising drug release pattern. The study provides a promising pathway for the development of a potent, sustained-release antifungal treatment based on natural products, with potential for further exploration and optimization.